Fig. 2. Elevated levels of miR-675 in OA chondrocytes. A, levels of miR-675-5' and 3' (A) and their host gene H19 (B), as well as let-7e (C) in freshly isolated chondrocytes from healthy and OA affected cartilage. miR levels were measured by TaqMan assays, and H19 by SYBR Green RT-qPCR. Statistical analysis was carried out with a non-paired, two-tailed t-test. N, non-OA; OA, osteoarthritis.